Document Number: 7365187
Abstract:
In a method for amplifying a DNA, comprising performing PCR using a sense primer and an antisense primer, PCR is performed in the presence of an additional sense primer comprising the sense primer and an oligodeoxyribonucleotide having a first additional sequence and ligated to the 5′ end of the sense primer and an additional antisense primer comprising the antisense primer and an oligodeoxyribonucleotide having a second additional sequence complementary to the first additional sequence and ligated to the 5′ end of the antisense primer; a Tm value of the additional sequences is lower than Tm values of the sense primer and the antisense primer; and annealing temperature in PCR is initially set to be a temperature at which the additional sequences do not anneal and changed in the course of PCR to a temperature at which the additional sequences anneal to each other.
Application Number: 10547354
Primary Class: 536 243
Sub-Classes: 435 6, 435 912
Inventors: Satoshi Hashiguchi, Kyoto, JP; Ken Inose, Kyoto, JP
Assignee: Arkray, Inc., Kyoto-shi, Kyoto, JP
Priority: JP: 2003-061841, 2003-03-07
PCT: WO PCT/JP2004/002896, 2004-03-05
Examiners: Young J. Kim (primary), David C. Thomas (assistant)
Filing Date: 2004-03-05
Publication Date: 2008-04-29
PDF: 7365187
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