Document Number: 20100077514
Abstract:
The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.
Application Number: 12626960
Primary Class: 800306
Sub-Classes: 536 231, 435419, 435412, 435416, 800298, 8003201, 800312, 435415, 800320, 800322, 8003203, 8003202, 536 241
Inventors: Hochul Yi, Cary, NC, US; Theodore M. Klein, Wilmington, DE, US; Joanne E. Barton, Wilmington, DE, US
Assignee: E.I. du Pont de Nemours and Company, Wilmington, US
Attorney, Agent or Firm: PIONEER HI-BRED INTERNATIONAL, INC., 7250 N.W. 62ND AVENUE, P.O. BOX 552, JOHNSTON, IA, 50131-0552, US
Provisional Application: US 60782671, 2006-03-15
Filing Date: 2009-11-30
Publication Date: 2010-03-25
PDF: 20100077514
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