Document Number: 20100192263
Abstract:
Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
Application Number: 12756211
Primary Class: 800298
Sub-Classes: 536 231, 435419, 435412, 435415, 435416, 435414
Inventors: Yumin Tao, Urbandale, IA, US; Dennis L. Bidney, Urbandale, IA, US; William J. Gordon-Kamm, Urbandale, IA, US; Leszek A. Lyznik, Johnston, IA, US
Assignee: Pioneer Hi-Bred International, Inc.
Attorney, Agent or Firm: PIONEER HI-BRED INTERNATIONAL, INC., 7250 N.W. 62ND AVENUE, P.O. BOX 552, JOHNSTON, IA, 50131-0552, US
Provisional Application: US 60700225, 2005-07-18
Filing Date: 2010-04-08
Publication Date: 2010-07-29
PDF: 20100192263
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